Multishot tomography for high-resolution in situ subtomogram averaging
Published in Journal of Structural Biology, 2023
Cryo-electron tomography (cryo-ET) and subtomogram averaging (STA) can resolve protein complexes at near atomic resolution, and when combined with focused ion beam (FIB) milling, macromolecules can be observed within their native context. Unlike single particle acquisition (SPA), cryo-ET can be slow, which may reduce overall project throughput. We here propose a fast, multi-position tomographic acquisition scheme based on beam-tilt corrected beam-shift imaging along the tilt axis, which yields sub-nanometer in situ STA averages.
Recommended citation: Sagar Khavnekar, William Wan, Parijat Majumder, Wojciech Wietrzynski, Philipp Erdmann, Juergen Plitzko, "Multishot tomography for high-resolution in situ subtomogram averaging." Journal of Structural Biology, 2023.
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