Optimizing Cryo-FIB Lamellas for sub-5Å in situ Structural Biology
Published in bioRxiv, 2022
We here present a method based on metallic platinum sputtering that can substantially enhance the quality of subtomogram averages from lamellas and thereby reduce the number of particles needed for high-resolution subtomogram averaging. We provide evidence for the physical background of this improvement and demonstrate its usefulness by producing sub-5Å ribosome averages from yeast.
Recommended citation: Sagar Khavnekar, Veronika Vrbovská, Magda Zaoralová, Ron Kelley, Florian Beck, Sven Klumpe, Abhay Kotecha, Jürgen Plitzko, Philipp Erdmann, "Optimizing Cryo-FIB Lamellas for sub-5Å in situ Structural Biology." bioRxiv, 2022.
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